Optimization of annealing temperature for amplification of the exon one region of the HPPD gene in HA1 accession sunflowers

Abstract The HPPD gene is one of the genes that actively involved in biosynthesis tocopherol, which main component Vitamin E. Sunflower raw materials for natural medicine since their oil contains vitamin To obtain complete information regarding whole sequences 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) sunflower accession HA1, exon region needs to be identified using a PCR-based method. One factor affecting success rate PCR annealing temperature. optimum temperature will produce specific target products. This study aimed determine optimal amplifying 1 research began with primer design activities, followed by vitro amplification (PCR), then optimization gradient PCR. results showed fragment was an 53 °C, two at 45 and three had 57 °C expected size products are 306 bp, 506 534 bp respectively.